Journal:

Article Title: Lipopolysaccharide from enterohemorrhagic Escherichia coli binds to platelets through TLR4 and CD62 and is detected on circulating platelets in patients with hemolytic uremic syndrome

doi: 10.1182/blood-2005-08-3219

Figure Lengend Snippet: LPS-induced platelet activation markers and binding of fibrinogen to human platelets detected by flow cytometry

Article Snippet: Detection of platelet activation was performed in resting platelets with hamster anti-mouse CD40L (10 μg/mL; BD Biosciences), followed by mouse anti-hamster-FITC (1:80; BD Biosciences).

Techniques: Activation Assay, Binding Assay, Flow Cytometry, Expressing

Journal:

Article Title: Lipopolysaccharide from enterohemorrhagic Escherichia coli binds to platelets through TLR4 and CD62 and is detected on circulating platelets in patients with hemolytic uremic syndrome

doi: 10.1182/blood-2005-08-3219

Figure Lengend Snippet: Expression CD40L on mouse platelets detected by flow cytometry

Article Snippet: Detection of platelet activation was performed in resting platelets with hamster anti-mouse CD40L (10 μg/mL; BD Biosciences), followed by mouse anti-hamster-FITC (1:80; BD Biosciences).

Techniques: Expressing, Flow Cytometry, Incubation

Journal: Immunity

Article Title: Low-Avidity CD4 + T Cell Responses to SARS-CoV-2 in Unexposed Individuals and Humans with Severe COVID-19

doi: 10.1016/j.immuni.2020.11.016

Figure Lengend Snippet:

Article Snippet: CD154-APC-Vio770 (clone: 5C8) , Miltenyi Biotec , Cat#130-114-130; RRID: AB_2751205.

Techniques: Functional Assay, Recombinant, Staining, Sequencing, Gene Expression, Software

Journal: International immunology

Article Title: Induction of Th1-biased cytokine production by alpha-carba-GalCer, a neoglycolipid ligand for NKT cells.

doi: 10.1093/intimm/dxq012

Figure Lengend Snippet: Fig. 3. Mechanisms of a-carba-GalCer-induced IFN-c production. (A and B) The levels of serum IL-12p70 and IFN-c after intravenous injection of a-GalCer (2 lg) (A) or a-carba-GalCer (2 lg) (B) were measured by ELISA or the cytometric bead array system at the indicated time points. Indicated antibodies were intraperitoneally injected 24 h before glycolipid injection. Data are means 6 SDs from three mice and repeated three times with similar results. (C and D) IL-12p70 production 6 h after intravenous injection of PBS, a-GalCer (2 lg) or a-carba-GalCer (2 lg) by spleen cells (C) and by splenic DC subsets (D). (C) Spleen cells were collected 1 h after glycolipid injection and cultured for 5 h in vitro. IL-12p70 production was analyzed by intracellular cytokine staining. (D) CD11c+ cells in (C) were further divided into CD8a CDCs, CD8a+ CDCs and B220+ PDCs, and IL-12p70 production was analyzed using intracellular cytokine staining. (E) CD40–CD40L interaction and IL-12 dependent production of IFN-c from NKTcells by a-carba-GalCer-pulsed BMDCs. Glycolipid-loaded or -unloaded BMDCs (2 3 105 cells per 200 ll) were co- cultured with NKT cells (7 3 104 cells per 200 ll) in the presence or absence of indicated mAbs (10 lg ml1). IL-12p70 and IFN-c in the supernatant at the indicated time point were measured by cytometric bead array. Results are representative of those from three independent experiments.

Article Snippet: For in vivo neutralizing experiments, goat polyclonal IgG specific for anti-mouse IL12 and anti-mouse CD40L was used and purchased from R&D systems (Minneapolis, MN, USA).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Cell Culture, In Vitro, Staining